platinum replica electron microscopy

Justin Taraska (NIH/NHLBI)
Project description:​
Book figure depicting workflow for Ni-NTA-Au labeled proteins. A platinum replica of the cell membrane is imaged and reconstructed via electron tomography.
Manuscript title:
Localizing proteins on single trafficking organelles in 3D with semisynthetic gold labeling and platinum replica electron microscopy
Membrane Trafficking. 2022 July; part of the Methods in Molecular Biology book series, volume 2473
PMID: 35819766
Illustrator: Ethan Tyler