|4/11/2023||Mengbi Yang, Amy Q. Wang, Elias C. Padilha, Pranav Shah, Natalie R. Hagen, China Ryu, Khalida Shamim, Wenwei Huang and Xin Xu||Division of Veterinary Resources||Article|
The Division of Veterinary Resources (DVR) continually supports the Intramural Research Program at the NIH, oftentimes behind the scenes of groundbreaking research. Two recent publications highlight DVR’s dedication and contributions. DVR was specifically recognized in the Acknowledgement sections of recently published work describing effective treatments against COVID-19. These were performed in Dr. Xin Xu’s lab with the National Center for Advancing Translational Sciences (NCATS). DVR’s very own Nida Delbe and Danitra Brathwaite, as well as the rodents we care for, were integral to the drug discovery now being investigated in humans. These studies include an oral formulation of Remdesivir1 and development of physiological based pharmacokinetic model in mice accurately predicting lung tissue concentrations in humans2.
|1/26/2023||William H. Herman ORCID logo ; Dean Schillinger; Shari Bolen; John M. Boltri; Ann Bullock; William Chong; Paul R. Conlin ORCID logo ; J. William Cook, IV; Ayotunde Dokun ORCID logo ; Naomi Fukagawa; Jasmine Gonzalvo ORCID logo ; M. Carol Greenlee;||Division of Library Services||Article|
The National Clinical Care Commission (NCCC) was established by Congress to make recommendations to leverage federal policies and programs to more effectively prevent and treat diabetes and its complications. The NCCC developed a guiding framework that incorporated elements of the Socioecological and Chronic Care Models. It surveyed federal agencies and conducted follow-up meetings with representatives from 10 health-related and 11 non–health-related federal agencies.
|4/8/2021||Li Jia||Division of Library Services||Article|
Adoptive T cell therapy (ACT) using ex vivo–expanded autologous tumor-infiltrating lymphocytes (TILs) can mediate complete regression of certain human cancers. The impact of TIL phenotypes on clinical success of TIL-ACT is currently unclear. Using high-dimensional analysis of human ACT products, we identified a memory-progenitor CD39-negative stem-like phenotype (CD39−CD69−) associated with complete cancer regression and TIL persistence and a terminally differentiated CD39-positive state (CD39+CD69+) associated with poor TIL persistence.
Other authors and affiliations: Sri Krishna, Frank J. Lowery, Amy R. Copeland, Erol Bahadiroglu, Ratnadeep Mukherjee, James T. Anibal, Abraham Sachs, Serifat O. Adebola, Devikala Gurusamy1, View ORCID ProfileZhiya Yu1, Victoria Hill, Jared J. Gartner, Yong F. Li, Maria Parkhurst, Biman Paria1, Pia Kvistborg, Michael C. Kelly, Stephanie L. Goff, Grégoire Altan-Bonnet, Paul F. Robbins, Steven A. Rosenberg
|4/1/2021||Candace Norton, Alicia A. Livinski||Division of Library Services||Article|
Bibliometric analyses are commonly used to measure the productivity of researchers or institutions but rarely used to assess the scientific contribution of national surveys/datasets. We applied bibliometric methods to quantify the contributions of the National Survey of Children's Health (NSCH) and the National Survey of Children with Special Health Care Needs (NS-CSHCN) to the body of pediatric health-related research. We also examined dissemination to nonresearch audiences by analyzing media coverage of statistics and research produced from the surveys.
|3/11/2021||Xizhong Cui, Junfeng Sun, Samuel J. Minkove, Yan Li, Diane Cooper, Zoe Couse, Peter Q. Eichacker, Parizad Torabi‐Parizi||Division of Library Services||Article|
Chloroquine (CQ) and hydroxychloroquine (HCQ) have been used as antiviral agents for the treatment of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV2) infection. We performed a systematic review to examine whether prior clinical studies that compared the effects of CQ and HCQ to a control for the treatment of non‐SARS‐CoV2 infection supported the use of these agents in the present SARS‐CoV2 outbreak.
|6/11/2020||Stephanie Chidester, Alicia A. Livinski, Anne F. Fish and Paule V. Joseph||Division of Library Services||Article|
This review was conducted to determine what is known from the literature about the effect of EV crosstalk on pancreatic β-cell function and viability in the pathogenesis of diabetes mellitus, to perform a gap analysis for future research directions, and to discuss implications of available evidence for diabetes care. The literature search yielded 380 studies from which 31 studies were determined to meet eligibility criteria.
|5/20/2020||Abhimanyu A. Amarnani, Katlin R. Poladian, Beatriz E. Marciano, Janine R. Daub, Sandra G. Williams, Alicia A. Livinski, Amy P. Hsu, Cindy L. Palmer, Cara M. Kenney, Daniele N. Avila, Steven M. Holland & James D. Katz ||Division of Library Services||Article|
This study characterizes rheumatological manifestations of GATA2 deficiency. Results: Rheumatological findings were identified in 28 patients, out of 157 cases reviewed (17.8%). Twenty-two of those patients (78.6%) reported symptom onset prior to or in conjunction with the molecular diagnosis of GATA2 deficiency.
|5/16/2019||Patricia Zerfas||Division of Veterinary Resources||Article|
During infection, hepatocytes must undergo a reprioritization of metabolism, termed metabolic reprogramming. Hepatic metabolic
reprogramming in response to infection begins within hours of infection, suggesting a mechanism closely linked to
This work not only addresses a key component of end-organ physiology, but also raises questions about the side effects of biologics in the treatment of inflammatory diseases.
|Macrophage Derived TNFa Promotes Hepatic Reprogramming to Warburg-like Metabolism|
|3/20/2019||Christopher W. Belter||Division of Veterinary Resources||Journal|
For 50 years, the Fogarty International Center (FIC) has built research capacity particularly in low and middle-income countries responding to national and global public health priorities.
FIC provides a bridge between NIH and the greater global health community by facilitating exchanges among investigators, providing training opportunities and supporting promising research initiatives in developing countries.
|Fifty Years of Supporting Global Health Research at the NIH FIC|
|12/6/2018||Patricia Zerfas||Division of Library Services||Article|
Given the central role of the liver in MMA pathophysiology, we generated a viable mouse model to study the acute and chronic hepatic disease manifestations. To recapitulate the acute-on-chronic metabolic crises intermittently experienced by MMA patients, we subjected these mice to a fasting challenge and explored changes in the hepatic transcriptome between the baseline and fasting states.
Among the many pathways differentially expressed between MMA mice and control littermates, a key metabolic regulator, Fgf21, emerged as most significant.
|FGF21 Underlies a Hormetic Reponse to Metabolic Stress in Methylmalonic Acidemia|
|3/28/2018||NINDS, Dr. Alexander Ksendzovsky||Medical Arts Branch||Journal||Neurology Volume 88, Number 13 |
|12/5/2017||Lynn Young||Division of Library Services||Journal|
Piezo2 is a mechanically activated ion channel required for touch discrimination, vibration detection, and proprioception.
Here, we discovered that Piezo2 is extensively spliced, producing different Piezo2 isoforms with distinct properties. Together, our results describe, at the molecular level, a potential mechanism by which transduction is tuned, permitting the detection of a variety of mechanosensory stimuli.
|Cell-Type-Specific Splicing of Piezo2 Regulates Mechanotransduction|
|11/13/2017||NINDS, Dr. Antonina Roll-Mecak||Medical Arts Branch||Journal||Nature Chemical Biology: Flavors of RNA imaging |
|11/3/2017||Josh A. Duberman||Division of Library Services||Article|
The focus of this chapter is on information for a variety of clinical research tasks, including searching for adverse effects, evidence-based practices, and research protocol development.
This chapter [also] presents an overview of information resources for clinical research, their scope and capabilities, in an attempt to demystify the process and results of searching. The purpose of this chapter is to provide a background framework on which to build and learn more.
|Information Resources for the Clinical Researcher |
|10/3/2017||Derek A. Newcomer ||Division of Occupational Health and Safety||Article|
Known originally for their use in nuclear air cleaning systems, high efficiency particulate air (HEPA) filters containing specialized media are now commonplace in the biomedical, aerospace, pharmaceutical, and clinical care settings.
As an engineering control, HEPA filters are the crux of the biological safety cabinet’s (BSC) performance and are essential for achieving filtration standards in clean rooms.
|8/1/2017||NHLBI, Dr. Ryan McGlinchey||Medical Arts Branch||Journal||Biochemistry Volume 56 |
|7/18/2017||NEI, Dr. Anthony St Leger ||Medical Arts Branch||Journal||Immunity Volume 47|
|7/15/2017||Patricia Zerfas||Division of Veterinary Resources||Article|
Mucolipidosis type IV (MLIV) is a lysosomal storage disease characterized by neurologic and ophthalmologic abnormalities. There is currently no effective treatment. MLIV is caused by mutations in MCOLN1, a lysosomal cation channel from the transient receptor potential (TRP) family.
In this study, we used genome editing to knockout the two mcoln1 genes present in Danio rerio (zebrafish). Our model successfully reproduced the retinal and neuromuscular defects observed in MLIV patients, indicating that this model is suitable for studying the disease pathogenesis.
|Novel Degenerative and Developmental Defects in a Zebrafish Model of Mucolipidosis Type IV|
|7/10/2017||NCI, Dr. Xin Wang ||Medical Arts Branch||Journal||Cancer Cell Volume 22 Number 1 |
|6/23/2017||NICHD, Dr. Garima Gupta||Medical Arts Branch||Journal||Endocrine Practice |
|6/6/2017||Patricia Zerfas||Division of Veterinary Resources||Article|
T cells undergo metabolic reprogramming with major changes in cellular energy metabolism during activation. In summary, the findings presented herein emphasize the role of COX particularly in T cells as a metabolic checkpoint for cell fate decisions following T cell activation, with heterogeneous effects in T cell subsets.
In addition, our studies highlight the utility of translational models that recapitulate human mitochondrial disease for understanding immunometabolism.
|Cytochrome c Oxidase Activity Is a Metabolic Checkpoint that Regulates Cell Fate Decisions During T Cell Activation and Differentiation.|
|5/23/2017||Josh Duberman||Division of Library Services||Article|
In this study, firstly, the length of time between patent application and approval of a new cancer drug is examined. For this purpose, the three most common cancer types - breast, prostate and lung cancer - were chosen. As part of this study it is clearly important to understand the pace of current basic biomedical research and to develop potential solutions to the obstacles and challenges that it faces.
With this in mind, in the second part of the study the reasons for this “time lag” in biomedical research are studied and defined in more detail.
|A Guide to Time Lag and Time Lag Shortening Strategies in Oncology-Based Drug Development|
|3/2/2017||Derek A. Newcomer ||Division of Occupational Health and Safety||Article|
Industrial hygiene practitioners are often consulted to recommend personal protective equipment (PPE) as a control strategy to minimize worker exposure to biological, chemical, and physical hazards.
When selecting laboratory coats, the industrial hygienist is concerned with limitations of dexterity, durability, employee comfort, and compatibility with the hazard of concern. These and other criteria provide the framework for an organization’s PPE program.
|The Last Line of Defense: Selecting the Right Laboratory Coat|
|1/4/2017||Verma Walker ||Division of Library Services||Journal|
The National Institutes of Health (NIH) Library ran a 3D-printing pilot to provide NIH staff with an opportunity to try out 3D-printing technology and to gauge their interest in a permanent 3D-printing service.
An additional goal of the pilot was to build partnerships among NIH scientists, fellows, students, engineers, and administrators who were interested in 3D printing, so that the library could serve as an information hub connecting individuals who have 3D printing needs to experts in 3D modeling and printing.
|Implementing a 3D Printing Service in a Biomedical Library |
|9/1/2016||NCI, Dr. Sam John ||Medical Arts Branch||Journal||Molecular Cell Volume 57 |
|7/15/2016||NCI, Dr. Christophe Marchand ||Medical Arts Branch||Journal||ACS Chemical Biology: The Community of Chemical Biologists |
|6/29/2016||NIAID, Dr. Daniel Green ||Medical Arts Branch||Journal||Cytokine & Growth Factor Reviews|
|6/2/2016||NCI, Dr. Ji Luo||Medical Arts Branch||Journal||Trends in Cancer |
|5/2/2016||Patricia Zerfas||Division of Veterinary Resources||Article|
In this paper we describe individuals with biallelic mutations in LAMA1, all of whom had the cerebellar dysplasia, myopia and retinal dystrophy, in addition to obsessive compulsive traits, tics and anxiety. Patient-derived fibroblasts have impaired adhesion, reduced migration, abnormal morphology and increased apoptosis due to impaired activation of Cdc42, a member of the Rho family of GTPases that is involved in cytoskeletal dynamics. LAMA1 knockdown in human neuronal cells also showed abnormal morphology and filopodia formation, supporting the importance of LAMA1 in neuronal migration, and marking these cells potentially useful tools for disease modelling and therapeutic target discovery.
|Cystic cerebellar dysplasia and biallelic LAMA1 mutations|
|2/1/2016||Patricia Zerfas||Division of Veterinary Resources||Journal|
Mutations in TRPML1 cause the lysosomal storage disease mucolipidosis type IV (MLIV). Most studies focus on the role of TRPML1 in constitutive membrane trafficking to and from the lysosomes. However, this cannot explain impaired neuromuscular and secretory cells’ functions that mediate regulated exocytosis.
Here, we analyzed several forms of regulated exocytosis in a mouse model of MLIV and, opposite to expectations, we found enhanced exocytosis in secretory glands due to enlargement of secretory granules in part due to fusion with lysosomes.
|Fusion of lysosomes with secretory organelles leads to uncontrolled exocytosis in the lysosomal storage disease mucolipidosis type IV.|